目的 对荨麻根的脂溶性成分进行研究。方法 采用硅胶、Sephadex LH-20、ODS柱色谱以及半制备HPLC等方法分离、纯化，根据化合物的理化性质和光谱数据鉴定结构。结果 从荨麻根乙酸乙酯部位分离鉴定了21个成分，分别为:(-)-荨麻醇(1)、(-)-开环异落叶松脂素(2)、23-羟基白桦脂酸(3)、2α， 3β， 24-三羟基-齐墩果-12-烯-28-羧酸(4)、臭矢菜素A(5)、二氢-4-羟基-5-羟甲基-2(3H)-呋喃酮(6)、绿原酸甲酯(7)、山柰酚(8)、松脂素单甲基醚-4′-O-β-D-葡萄糖苷(9)、罗汉松脂素-4′-O-β-D-葡萄糖苷(10)、环橄榄脂素-6-O-β-D-吡喃葡萄糖苷(11)、豆甾醇-3-O-β-D-葡萄糖苷(12)、烟酰胺(13)、反式咖啡酸-4-O-β-D-葡萄糖苷(14)、七叶苷(15)、5-羟基-7-甲氧基香豆素-8-O-β-D-葡萄糖苷(16)、6-甲氧基-木犀草素-7-O-β-D-葡萄糖苷(17)、木犀草素-7-O-β-D-葡萄糖苷(18)、槲皮素-3-O-(4″-甲氧基)-α-L-吡喃鼠李糖苷(19)、2′-脱氧尿苷(20)和芹菜素-6， 8-二-C-β-D-葡萄糖苷(21)。结论 除化合物8、12外，其余19个成分均为首次从荨麻中分得;化合物5、6、9、10、11、14、16、17、19均为首次从荨麻科植物中发现。
Abstract
OBJECTIVE To investigate the liposoluble constituents of Urticae Rhizoma. METHODS The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS gel column chromatographies, and semi-preparative HPLC. The structures were elucidated on the basis of spectral data and physiochemical properties. RESULTS Twenty-one compounds were isolated from the ethyl acetate fraction of Urticae Rhizoma, and identified as(-)-urticol(1),(-)-secoisolariciresinol(2), 23-hydroxybetulinic acid(3), 2α,3β, 24-trihydroxy-12-oleanen-28-oic acid(4), cleomiscosin A (5), dihydro-4-hydroxy-5-hydroxymethyl-2(3H)-furanone(6), methyl chlorogenate(7), kaempferol(8), pinoresinol monomethyether-4′-O-β-D-glucopyranoside(9), martairesinol-4′-O-β-D-glucopyranoside(10), cycloolivil-6-O-β-D-glucopyranoside(11), stigmasterol-3-O-β-D-glucopyranoside(12), nicotinamide(13), trans-caffeic acid-4-O-β-D-glucopyranoside(14), esculin(15), 5-hydroxyl-7-methoxycoumarin-8-O-β-D-glucopyranoside(16), 6-oxymethyluteolin-7-O-β-D-glucopyranoside(17), luteolin-7-O-β-D-glucopyranoside(18), quercetin-3-O-(4″-methoxy)-α-L-rhamnopyranoside(19), 2′-deoxy uridine(20), and apigenin-6, 8-di-C-β-D-glycoside(21), respectively. CONCLUSION All the compounds, except 8 and 12, are isolated from U. fissa for the first time. Meanwhile, compounds 5, 6, 9, 10, 11, 14, 16, 17, and 19 are all found in Urticaeae plants for the first time.
关键词
荨麻 /
根茎 /
脂溶性成分 /
结构鉴定 /
(-)-荨麻醇 /
(-)-开环异落叶松脂素
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Key words
Urtica fissa /
rhizome /
liposoluble constituent /
structure identification /
(-)-urticol /
(-)-secoisolariciresinol
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中图分类号：
R284
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脚注
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基金
国家自然科学基金资助项目(81374067)
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